diff --git a/tests/test_telegram_polling_progress_ptb.py b/tests/test_telegram_polling_progress_ptb.py
index c25c50335820..1eab796354cd 100644
--- a/tests/test_telegram_polling_progress_ptb.py
+++ b/tests/test_telegram_polling_progress_ptb.py
@@ -92,6 +92,34 @@ class _EnvelopeRequest(BaseRequest):
         return 200, self.payload
 
 
+class _SlottedEnvelopeRequest(BaseRequest):
+    """A getUpdates request with no instance ``__dict__``.
+
+    Reproduces PTB's real HTTPXRequest shape on Python 3.13, where every
+    class in the MRO defines ``__slots__`` and instances therefore reject an
+    instance-attribute ``do_request`` monkey-patch as "read-only" (#64482).
+    ``__slots__`` names the payload so the double needs no ``__dict__``.
+    """
+
+    __slots__ = ("_payload",)
+
+    def __init__(self, payload):
+        self._payload = payload
+
+    @property
+    def read_timeout(self):
+        return 10
+
+    async def initialize(self):
+        return None
+
+    async def shutdown(self):
+        return None
+
+    async def do_request(self, url, method, request_data=None, **_kwargs):
+        return 200, self._payload
+
+
 async def _cancel_task(task):
     if task is None or task.done():
         return
@@ -226,6 +254,43 @@ async def test_real_base_request_valid_success_envelope_records_progress():
     assert adapter._send_path_degraded is False
 
 
+@pytest.mark.asyncio
+async def test_slotted_request_instrumented_without_read_only_error():
+    """Instrumenting a __slots__ request must not raise, and must still record.
+
+    Regression for #64482: PTB's HTTPXRequest carries no instance ``__dict__``
+    on Python 3.13, so the old ``request.do_request = wrapper`` monkey-patch
+    raised ``AttributeError: ... 'do_request' is read-only`` and broke every
+    Telegram connect. The subclass re-tag must instrument such an instance and
+    still observe getUpdates progress.
+    """
+    adapter = TelegramAdapter(PlatformConfig(enabled=True, token="123456:test-token"))
+    generation, progress = adapter._begin_polling_generation()
+    adapter._polling_network_error_count = 4
+    adapter._polling_conflict_count = 3
+
+    request = _SlottedEnvelopeRequest(b'{"ok":true,"result":[]}')
+    # Guard the premise: the slotted instance rejects the old monkey-patch,
+    # exactly as PTB's HTTPXRequest does on Python 3.13.
+    with pytest.raises(AttributeError, match="read-only"):
+        request.do_request = lambda *a, **k: None
+
+    instrumented = adapter._instrument_polling_request(request)
+    context_token = tg_adapter._POLLING_GENERATION_CONTEXT.set(generation)
+    try:
+        result = await instrumented.post(
+            "https://api.telegram.org/bot-token/getUpdates"
+        )
+    finally:
+        tg_adapter._POLLING_GENERATION_CONTEXT.reset(context_token)
+
+    assert result == []
+    assert progress.is_set()
+    assert adapter._polling_network_error_count == 0
+    assert adapter._polling_conflict_count == 0
+    assert adapter._send_path_degraded is False
+
+
 @pytest.mark.asyncio
 async def test_real_ptb_stop_cleanup_cannot_heal_recovery_generation():
     assert tg_adapter.TELEGRAM_AVAILABLE is True